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For fractionation of cells into cytosolic, membrane and nuclear fractions, we used a digitonin fractionation method that was described previously.
Importantly, this confirmed that the cell fractionation method generated a pure CM fraction with minimal NM contamination.
To further verify these results, we used an additional fractionation method, which separates cell membrane fractions on a continuous OptiPrep/sucrose density gradient.
An aerosol size fractionation method was used to quantify the fraction of metal captured.
Lysosomes are usually co-fractionated in the mitochondrial pellet in this fractionation method.
Synaptophysin was also measured in the samples as a control for the fractionation method (as the membrane-bound synaptophysin should appear predominantly in the TX fraction) and as a surrogate marker for synaptic density/neuron loss.
Suzuki, K., Bose, P., Leong-Quong, R. Y., Fujita, D. J. & Riabowol, K. REAP: a two minute cell fractionation method.
We have also attempted to apply his fractionation method to study sub-cellular distribution of substance P in the nervous system.
Isolation and purification of mitochondria was performed by optimized cell fractionation method.
A continuous fractionation method for mixed particulate solids has been developed.
Our new fractionation method and the Heithaus stability evaluation method were applied to hydroprocessed residual oils.
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