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At the end of the focusing period, ten fractions from each sample were collected and 5 µL of each fraction was subjected to SDS-PAGE for analysis of fractionation efficiency.
More importantly, the SAX-based digestion procedure did not influence the SAX-based peptide fractionation efficiency which was done in the same SAX membrane.
Pore size also strongly influences the fractionation efficiency.
Initial moisture content (MC) is the principal factor governing steam consumption and affecting the fractionation efficiency [ 25, 26].
Fractionation efficiency was assessed by immunoblot using an antibody to anti-GAPDH (cytoplasm) and anti-histone H4 (nuclear (chromatin-bound)).
During the ethanol extraction process, 0.1 to 0.5% (w/w) HCl or NaOH was added in order to improve the lignin fractionation efficiency from SEWS.
Similar(53)
TP of the three FFD-SF operations were 37.3, 22.1, and 17.9 kg/h, and the fractionation efficiencies (FE) of the four size fractions were 80.5, 73.7, 79.1 and 86.1%.
Differences in transfection and fractionation efficiencies were corrected for by analysing VA and GAPDH RNA levels (see Materials and methods).
This method outweighs previous post-synthesis fractionation methods in efficiency and scalability; thus it is more promising for large-scale synthesis of size-controlled GO.
Fractionation and transfection efficiency was analysed by detection of co-transfection control VA or EPO RNA as well as endogenous GAPDH mRNA.
The presence of COX IV only in the mitochondrial fraction and α-tubulin only in the cytosolic fraction proved the efficiency of the fractionation.
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