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The 5 10 and <1 kDa fractions were selected for further fractionation by gel filtration chromatography.
Fractionation by anion exchange gave fractions with lower activity compared to cation exchange fractionation (Table S7).
Fractionation by cation exchange resulted in several fractions with strong protease activity (Table S6).
Following the protocols of our laboratory we performed a primary fractionation by open column chromatography to afford 37 fractions.
Serial fractionation by ion-exchange chromatography produced two main fractions differing in the monosaccharide composition and sulfate content [10].
We confirmed the fractionation by Coomassie staining the cytosolic and chromatin fractions and by tubulin immunostaining.
On fractionation by gel-filtration, inhibitory activity was found in fractions of higher molecular weight than IgG.
After fractionation by ultracentrifuge, His6-PrS2-Bac7 (1–35) was fully recovered in the soluble fraction (Fig. 1b).
The introduction of tissue fractionation by Albert Claude allowed mitochondria to be isolated from other cell fractions and biochemical analysis to be conducted on them alone.
It is the first example of copolymer fractionation by CPF.
Table 9 summarizes the results of the fractionation by open column chromatography.
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