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The fulvic fraction (acid soluble) was fractionated through a column of Amberlite XAD-7 resin.
To purify small RNA, total RNA was fractionated through a flashPAGE Fractionator (Applied Biosystems), and small RNA was retrieved.
Cytoplasmic extracts were fractionated through sucrose gradients, with the lightest components appearing at the top (fractions 1 and 2), small (40S) and large (60S) ribosomal subunits, and monosomes (80S) in fractions 3 6, and progressively larger polysomes in fractions 7 12.
The polysaccharides were fractionated through ion-exchange chromatography, and were shown to be homogeneous via high-performance size-exclusion chromatography (HPSEC).
Curdlan ((1 → 3 -β-D-glucan) was found to be fractionated through liquid—liquid phase equilibrium with the mixture dimethyl sulfoxide + lithium chloride as a solvent and acetone as a precipitant.
Approximately, 20µg of total RNA was fractionated through a 15% polyacrylamide-Urea gel (Sequagel, National diagnostic) along with 32-P labeled 19 24bp oligonucleotide-delimiting markers.
Similar(20)
Fifty micrograms of protein was size-fractionated through a 10% SDS-PAGE gel and transferred onto nitrocellulose filters.
The lysates were boiled, size-fractionated through 15% SDS PAGE, and then were transferred onto a polyvinylidene-difluoride membrane (Millipore, Bedford, MA, USA).
Briefly, double stranded cDNA was synthesized from poly(A) RNA, size-fractionated through a Sepharose CL-2B gel filtration column, and ligated into λ Uni-ZAP XR vector.
Particles <40 μm were size-fractionated through filters (nylon, Millipore, Darmstadt, Germany) to obtain different plankton size classes: (i) 20 40 μm, (ii) 2 20 μm and (iii) <2 μm.
PilJ is part of the chemosensory systems of P. aeruginosa [ 45], and it was likely co-fractionated through its association with another component of the chemosensory system present in the outer membrane.
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