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The methanol extract of P. fernandopoioana was not fractionated since it showed no activity.
Following crude extracts chemical and biological analysis, the dichloromethane extract from leaves (1st collection) was chosen to be fractionated, since it showed to be the most active against the three Mycobacterium tuberculosis strains (RMPr, H37Rv and INHr) (Table 2).
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Gradients were fractionated with fraction collector Model 2128 (Biorad).
The polymers were fractionated by fractional precipitation.
Since methylene chloride extracted the most active ingredients from the methanol/water extract of A. altissima, our focus was shifted to Fraction C. Fraction C was further fractionated into C1, C2, C3 and C4 using preparative TLC.
The CsCl gradient was fractionated into 22 fractions through bottom puncture of the centrifugation tubes.
Active fractions were further fractionated and assayed.
CSF peptides were fractionated into 30 fractions.
Fraction S14 was further fractionated by RP-HPLC.
Since the crossed DNA segments can either be G- or T-segment by chance, they are fractionated into both IP fractions, P1 and P2.
Gradients were again fractionated, and the virus containing fractions identified by gel electrophoresis.
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