Relatively small size differences can be resolved, such that log-phase cell cultures can be fractionated into populations of cells at particular phases of the cell cycle [22], presumably with the proviso that the cell cycle phase correlates well with cell size for the cell line used.
The level of sensitisation within spheroid sub-populations correlated with HIF-1 activity determined using fractionated sub-populations from HCT116 cells expressing an HIF-1 responsive-luciferase reporter.
Lamellar bodies were isolated from rat lungs, separated into limiting membrane and core populations, fractionated by SDS-PAGE and proteins identified by nanoLC-tandem mass spectrometry.
Brain mitochondria were fractionated into different populations for genotyping to confirm that there was no mtDNA heterogeneity in hypervariable regions that might be associated with metabolic heterogeneity.
This choice was grounded on Alam et al.'s study [ 15] that confirmed the significant content of the α1 subunit in PBMCs and fractionated T-cell populations.
We assessed the mammosphere formation of sorted CD44high/CD24low or ALDHhigh cells that were further fractionated from either population C (Ep-CAMhigh/CD49fneg) or population B (Ep-CAMhigh/CD49f+) cancer cells.
To investigate the actual imprinting status of the genes expressed in adult blood, we genotyped common expressed single nucleotide polymorphisms (SNPs) in a cohort of 50 healthy adults and analysed allelic expression in both whole blood and fractionated cellular sub-populations.
In the stem-like (BCRP+) cell population fractionated from RB143 human retinoblastoma cells, miR-328, miR-519c, and miR-520h levels are found to be lowered 9-, 15-, and 3-fold, respectively, suggesting that the high level of BCRP expression is regulated by these miRs [217].
Accordingly, WT CD4+CD25− and CD4+CD25+ T cell populations were fractionated using magnetic beads to >90% purity, and 1×106 cells of each fraction were cultured in the presence of DBTC (3 µM, dissolved in ethanol) or ethanol carrier alone (the final concentration of ethanol in the culture medium was 0.1%).
Finally, within the fractionated cell populations, there was evidence for coexpression of lineage specific markers alongside of pluripotency genes, similar to lineage priming described first in hematopoietic stem cells [15].
