Composted PL samples were fractionated into readily plant-available P, labile inorganic P (another plant-available fraction), sesquioxide-associated (Fe oxide and Al oxide) inorganic P- and Ca-associated P by sequential extraction with de-ionized water, 0.5 M NaHCO3 (pH 8.5), 0.1 M NaOH, and 1 M HCl.
Leaf homogenate extracts from Zera-EGF transgenic tobacco plants were fractionated on density gradients and analyzed by gel electrophoresis and immunoblotting using anti-Zera and anti-BiP antibodies.
Gradients were fractionated with fraction collector Model 2128 (Biorad).
To evaluate precise activity of the plant, VRM was fractionated successively with petroleum ether, chloroform, ethyl acetate, water, and the resulting fractions were tested similarly.
Ajuga integrifolia, a plant with better antimalarial effect, was further fractionated using solvent fractionation technique [ 23].
The same amount of WT tobacco plant leaves were fractionated and analyzed simultaneously as a negative control.
Leaves of the plant were successively fractionated in various organic solvents.
The active crude plant extract was fractionated first by solvent partitioning and then by HPLC.
The samples were then centrifuged for 2 min at 4°C, 14,000 g, and aliquots that derived from 2.5 mg plant powder were fractionated by SDS-PAGE and blotted on a PVDF membrane (Bio-Rad).
Even though this investigation supports the traditional medicinal usage in the Ethiopian community and the medicinal uses of these plants in different regions of Ethiopia; to obtain the most promising antimycobacterial activity and to conclude their anti-TB activity, these plants should be fractionated with less polar solvents.
