Sentence examples for fractionated material from inspiring English sources

We separated an oxidized IgG1 monoclonal antibody based on the oxidation state of methionine 252 and analyzed fractionated material in receptor binding experiments as well as in functional (cell-based) assays.

As expected, no SHL8 activity was detected in HPLC fractionated material translated from EBNAγ mRNA encoding SHL8 in the 3' region.

Comparison of the bioactivity of the unseparated and fractionated material revealed that pooled fractions 3 and 4 showed a comparable strong stimulatory capacity as the starting material and that the following chromatographic fractions exhibited a gradual decline in biological activity.

Both the fractionated material used to generate the SSH library and that used to screen the library was validated for fractionation into adipocyte and stromal fractions based on expression of marker transcripts for these two fractions.

Approximately 1 3 mg of wall residue or fractionated material was hydrolyzed (2 M TFA, 110°C, 2 h) and the liberated monosaccharides converted to methoxy sugars using 1 M methanolic HCl at 80°C for 24 h.

This amount of HA-FUSWT in the cytoplasmic FUS was similar to that seen when endogenous HEK293T cells were fractionated (Supplementary Material, Fig. S3).

The shape and size of the Aβ band in Western blotting of the size-fractionated material and the immuno-purified peptide were the same, which suggested that the process did not selectively enrich any of the Aβ peptides.

Preliminary characterization of the non-fractionated materials showed that a low difference in their average tacticity (PP2>PP1) leads to an important modification of their rigidity properties.

The beads were then washed with the same buffer and the bound (and input) material fractionated on an SDS polyacrylamide gel and detected with a Typhoon PhosphorImager (Amersham).

Samples were spun for 15 min at 20,000 x g to remove insoluble material, and the solubilized material was fractionated by gravity flow over a 1 ml Sephacryl-S-300 column pre-equilibrated in column buffer (50 mM HEPES [pH 7.5], 200 mM KOAc, 15 mM MgOAc2, 1 mM DTT, and 0.25% digitonin).

The gas and particulate phase material was fractionated by HPLC into nonpolar, moderately polar and highly polar chemical fractions.