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This range was fractionated into three fractions and ACE-I inhibitory activity determined for each.
P. dasyphylla crude extract was fractionated into three fractions using different range of solvents (water, ethyl acetate and hexane) whereby all the fractions were tested for its hyaluronidase inhibitory activity.
Bulk soil was fractionated into three aggregate size classes (> 2000 μm large macroaggregates; 2000 250 μm small macroaggregates; < 250 μm microaggregates) by optimal-moisture sieving.
The cDNA library was fractionated into three sets of cDNAs containing large, medium and small fragments.
Each cDNA library was then fractionated into three sets containing small, medium and large fragments using columns provided by the manufacturer.
After the 21-d equilibration, the two spiked sediments were fractionated into three sizes, i.e., sand (53 2000 μm), silt (2 53 μm), and clay (<2 μm) by using a combination of wet sieving and centrifugation (Stemmer et al. 1998).
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The methylene chloride extract (Fraction C) was further fractionated into four fractions (C1 C4) of different Rf values using preparative thin layer chromatography (TLC) plates (0.2 mm, POLYGRAM® SIL G/UV254, Macherey-Nagel GmbH & Co, Duren, Germany) and a solvent system: acetone:ethyl acetate = 1 4 (v/v).
The alloy was fractionated into four fractions by temperature-gradient fractionation, and the fractionation was analyzed by 1H NMR, 13C NMR, DSC and WAXD.
Particularly, sample B exhibits better toughness, higher molecular weight and slight lower flexural properties than sample A. Then, these two samples were fractionated into six fractions via temperature rising dissolution fractionation, respectively.
The crude PEP was fractionated into two fractions, namely PEP-1 and PEP-2.
The natural organic matter (NOM) from the sediment elutriate, which was obtained from a drinking water reservoir in South China, was fractionated into four fractions by using XAD-8 and XAD-4 resin.
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