The polymers were fractionated by fractional precipitation.
The gradients were fractionated using a piston gradient fractionator (BioComp Instruments, Fredericton, NB, Canada) and UV absorbance at 254 nm was monitored by a UV-Monitor (BioRad, Hercules, CA).
The gradients were fractionated using a piston gradient fractionator (BioComp Instruments, Fredericton, NB, Canada) and UV absorbance at 254 nm was monitored using a UV-Monitor (BioRad, Hercules, CA).
Active fractions were further fractionated and assayed.
CSF peptides were fractionated into 30 fractions.
Fraction S14 was further fractionated by RP-HPLC.
Following centrifugation, gradients were fractionated using a BioComp gradient station model 153 (BioComp Instruments, New Brunswick, Canada) measuring cytosolic RNA at 254 nm.
Briefly, isobaric-labeled peptides obtained from biological replicate 1 were fractionated on a TSKGel Amide 80 HILIC-HPLC column by using the Agilent 1200 microHPLC instrument.
RNA products were fractionated on 15% polyacrylamide/8.3 m urea gels, stained with SYBR Gold (Invitrogen) and imaged with a Typhoon FLA 9500 instrument (GE/Fujifilm).
Trypsin digested samples were fractionated offline by high pH reverse phase chromatography [ 47] using a MAGIC 2002 high pressure liquid chromatography (HPLC) instrument (Michrom BioResources, Inc., Auburn, CA) and C18 Gemini-NX column [150 mm × 2 mm i.d., 5 um particle, 110 Å pore size (Phenomenex, Torrence, CA)].
