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Fractionated heart (control or hydrogen peroxide treated) samples prepared for Western blot analysis were probed with polyclonal antibodies against Prdx1, Prdx2, Prdx3, or hyperoxidized Prdx.
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We suspect this could well stem from differences in methodology, as the mouse hearts were fractionated into their subcellular components prior to analysis, which would favor identification of lower abundance proteins from the cytosol and membranes.
Gradients were fractionated with fraction collector Model 2128 (Biorad).
The polymers were fractionated by fractional precipitation.
The CsCl gradient was fractionated into 22 fractions through bottom puncture of the centrifugation tubes.
Active fractions were further fractionated and assayed.
CSF peptides were fractionated into 30 fractions.
Fraction E was further fractionated by silica gel CC eluting with a gradient of CHCl3 MeOH (50:1–10:1) to yield five fractions (E1-E5).
Fraction S14 was further fractionated by RP-HPLC.
Gradients were again fractionated, and the virus containing fractions identified by gel electrophoresis.
The gradients were fractionated into 38 1-mL fractions.
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