Sentence examples for fractionated from the from inspiring English sources

Each 1 ml fraction was carefully fractionated from the centrifuge tubes and was observed by fluorescence microscopy to find MFIB-containing mitochondria.

Gradients were fractionated from the bottom (1 ml fractions) and analyzed for PABP, B5 or ICP4 by Western blotting.

After centrifugation for 18 h at 100,000 g, gradients were fractionated from the top into five fractions of equal volume (with the solubilized pellet as the bottom fraction) and analyzed by SDS-PAGE, using a Tris-Tricine buffer system [65] and phosphorimaging using a Storm 840 phosphorimager and allied software (GE Healthcare Life Sciences, Piscataway, NJ).

At the end of the run, the gradient was fractionated from the top into 250 μl fractions.

The gradients were fractionated from the top manually by pipetting into ∼1 ml fractions.

Four components (P1 P4) with different molecular size were fractionated from the conjugate.

Actin was first polymerized in vitro, and then G-actin (supernatant fraction S) fractionated from F-actin (pelleted fraction P) by centrifugation at 150,000 X g for 1.5 hours.

Where required, Treg-phenotype cells and residual Teff cells were fractionated from SMCs with the CD4+CD25+ Treg-cell isolation kit (Miltenyi Biotech), and each fraction set at 0.5 × 10 cells/mL, either alone or together, for co-culture with BM-derived dendritic cells as a source of APC at 1.25 × 10 cells/mL.

Twelve samples extracted and fractionated from SZ, DS and the formula (SZ+DS) were analyzed.

PolyA+ mRNA were then fractionated from total RNA using the NucleoTrap mRNA Kit (BD Biosciences Clontech, Palo Alto, CA, USA) which combines a spin-column filter with oligo (dT) latex bead technology.

Regional lymph nodes were removed and cells were separated by mincing the tissue and disaggregating it through a needle of 22 G. PBMNC from naïve rats, AIA rats and IB-MECA-treated rats were fractionated from heparinized blood using the Ficoll Hypaque gradient.