Sentence examples for fractionated from from inspiring English sources

Actin was first polymerized in vitro, and then G-actin (supernatant fraction S) fractionated from F-actin (pelleted fraction P) by centrifugation at 150,000 X g for 1.5 hours.

Each 1 ml fraction was carefully fractionated from the centrifuge tubes and was observed by fluorescence microscopy to find MFIB-containing mitochondria.

Gradients were fractionated from the bottom (1 ml fractions) and analyzed for PABP, B5 or ICP4 by Western blotting.

After centrifugation for 18 h at 100,000 g, gradients were fractionated from the top into five fractions of equal volume (with the solubilized pellet as the bottom fraction) and analyzed by SDS-PAGE, using a Tris-Tricine buffer system [65] and phosphorimaging using a Storm 840 phosphorimager and allied software (GE Healthcare Life Sciences, Piscataway, NJ).

At the end of the run, the gradient was fractionated from the top into 250 μl fractions.

The gradients were fractionated from the top manually by pipetting into ∼1 ml fractions.

Ribosome-associated mRNAs were then fractionated from cytoplasmic extracts into two pools: an under-translated pool (fraction-U) containing mRNAs with four or less ribosomes and a well-translated pool (fraction-W) containing mRNAs with five or more ribosomes.

To confirm the presence of MYOC aggregates in the transgenic flies, we fractionated homogenates from fly heads into soluble and insoluble fractions.

To verify PspA secretion, we fractionated cells from each strain and determined the level of PspA in each fraction.

One way to do this would be through disproportionation reactions that yielded fractionated products from an intermediate oxidation state reactant.

We fractionated CM from stimulated and control cells using sequential centrifugation and collected microparticles in the pellet at 100,000×g (100K) fraction [28], [29].