Exact(4)
The SOM was fractionated following procedures described by [25].
Postmortem human PFC tissues were fractionated following the protocols of Carlin et al [25] and Phillips et al [14], [26], as well as by combining these two methods with some modifications (named Method 1, Method 2 and Method 3 respectively).
The flow-through fraction from the immunodepletion was denatured by adding 0.48 g/ml urea and 13 µl/ml neat acetic acid and the samples were injected onto a macroporous Reversed-Phase (mRP) C18 Column (4.6×50 mm, 5188-5231, AgilenTechnologieses) and fractionated, following recommendations by the manufacturer.
cDNA was size fractionated following manufacturers instructions.
Similar(56)
The urine proteins of patients with UTUC were fractionated into four fractions following the same procedures for that of healthy people.
They measured a greater decrease of pO2 and a greater area of necrosis following fractionated irradiation.
We observed an upregulation of some anti-apoptotic proteins mainly with Bcl-XL in both situations following fractionated radiation.
Afterwards, the ethanol extract was fractionated with the following solvents: petroleum ether (1.5 L); chloroform (2 L); ethyl acetate (2 L); ethanol (2 L) and water (2 L) (aqueous fraction).
Since two of four cell lines exhibiting heightened responsiveness to CD95-mediated signals following fractionated irradiation express mutant p53 and displayed little or no up-regulation of CD95, enhanced responsiveness did not correlate with p53 status and CD95 up-regulation.
Surviving fractions were determined following fractionated irradiations of 1.0 to 5.0 Gy each per fraction with interfractional time intervals of 4 hr.
PLD-repair following fractionated irradiation was studied by giving tumours seven fractions of 2.0 Gy over 7 days and then, after an interval of 24 h, single graded radiation doses in the range 6-21 Gy.
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