Sentence examples similar to fractionated by two from inspiring English sources

The supernatants were fractionated by SDS-12.5% PAGE, transferred to Immobilon-P PVDF membranes (Millipore, Billerica, MA), and analyzed by immunoblotting (IB) with antibodies indicated in specific figures.

In Methods 2 and 3, the SPM was further fractionated by Triton X-100 extraction first at pH 6, then at pH 8 (Figure 1A).

Each cultivar was fractionated by hand into two fractions: leaf (leaf blade and leaf sheath) and stem (internodes and nodes) and weighed.

The RPHPLC fraction of E5PcF3 was further fractionated by SEC into seven fractions (C1 to C7), as shown in Figure  1.

The LC program included a 5-min linear gradient from buffer A (50% acetonitrile with 0.1% formic acid) to buffer B (99.9% acetonitrile with 0.1% formic acid), a constant elution in buffer B for 10 min, followed by a linear gradient return to buffer A in 5 min. Samples were fractionated by a 2.1×50 mm Eclipse Plus-C18 column (Agilent) with a flow rate of 0.5 ml min−1.

Active RP fractions were further fractionated by normal-phase (NP) LC into eight fractions with increasing polarity.

Following precipitation, the samples were fractionated by PEI cellulose in two dimensions.

Prior to analysis, this sample was homogenized and fractionated by ultracentrifugation resulting in four subcellular fractions: cytosol, mitochondria/nuclei, endoplasmatic reticulum/golgi and plasma membranes.

However, when fractionated by SDS-PAGE all three FLNs migrate at approximately 280 kDa, in a region where there are few other proteins, meaning that a rough approximation of total FLN content can be obtained by protein staining.

The polymers were fractionated by fractional precipitation.

Fraction DS4 was further fractionated by size exclusion chromatography (Sephadex G-75) and three fractions were collected (DS4G1 through DS4G3)).