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The polymers were fractionated by fractional precipitation.
Fraction S14 was further fractionated by RP-HPLC.
Gradients were fractionated by a Piston Gradient Fractionator (BioComp).
Cells were fractionated by differential centrifugation for the cytoplasmic and nuclear fractions.
Isolated fractions were dried by vacuum centrifugation, digested with trypsin, and fractionated by reverse phase HPLC.
Extracts were fractionated by C18 reverse-phase solid phase extraction.
The fluid was also fractionated by molecular filtration.
Non-axenic soil was fractionated by buoyant density centrifugation.
Saturate, aromatic, and polar compounds were fractionated by n-hexane, benzene, and chloroform, respectively.
Protein (30 μg) was fractionated by SDS-polyacrylamide gel electrophoresis and then electroblotted onto nitrocellulose membranes.
Stable isotopic compositions of water are constantly fractionated by temperature, season and evaporation effects.
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