To identify the source of testis S-2HG, we fractionated testis extracts and identified the fractions that actively produced S-2HG.
To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA.
Compounds in the lipid classes fractionated were diluted in isopropanol and identified using a Micromass Q-TOF micro system (Waters Co., UK) equipped with a syringe pump, LockSpray and ESI interface, operated in positive ionization mode.
Additional studies should be carried out to isolate and identify the bioactive compounds in the fractionated water extract, in order to provide more convincing evidence.
Previously, DCOE has been fractionated into four major fractions and various constituents of each fraction were identified and their in vitro and in vivo antioxidant activities were evaluated (Shebaby, et al. 2013, submitted).
To evaluate the genotoxicity and identify the mutagens in the soil before and after the various treatments, fractionated extracts of five soils were bioassayed for mutagenic activity with a microsuspension modification of the Salmonella histidine reversion assay.
The fractionated lipids were identified from the rate-of-flow values of the ceramide standards and quantified by density measurements with a CS Analyzer version 2.0 (Atto, Tokyo, Japan).
The identities of the fractionated peaks were identified by gas chromatography (GC) or GC/mass spectrometric (MS) analyses after derivatization (e.g., acid methanolysis) [3], [12].
Lamellar bodies were isolated from rat lungs, separated into limiting membrane and core populations, fractionated by SDS-PAGE and proteins identified by nanoLC-tandem mass spectrometry.
In this work, the mass culture of T. vaginalis was fractionated to identify sphingomyelinase activity present in the total extract (TE) and the subcellular fractions P30 and S30.
