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This shows that 3He/4He ratios are also fractionated along with Ne, suggesting the presence of residual deep-implanted solar He and Ne after loss of solar gases implanted in shallow depth of the particles.
This set of synthetic mRNAs fractionated along with the human brain mRNA gives us a series of profiles with known and consistent characteristics to assist in comparing the data between the three separate length fractionations.
Briefly, approximately 600 µg of total cell RNA prepared from HPV16-positive CaSki C-2 cells [a CaSki subclone cell line [72] was dissolved in RNA loading buffer II (Ambion, TX) and denatured at 75°C for 5 min. The RNA samples were then fractionated, along with 32P-labeled RNA oligos as size markers, in a 15% denaturing polyacrylamide gel containing 8 M urea.
This finding suggests that mrPFC might be functionally fractionated along a dorsal ventral gradient.
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The polymers were fractionated by fractional precipitation.
To take equimolar concentrations of the protein, bacterial extracts containing NF-L and MBP-tagged Myo Va proteins were fractionated on gels along with known concentrations of BSA.
Protein A/G Sepharose beads were added and incubated for another 1 hr at RT. Immunocomplexes were centrifuged and the precipitates were washed (3X) with high salt solution (50 mM Tris-HCl, pH 7.4; 500 mM NaCl; 1 mM EDTA; 0.1% NP40; 0.02% SDS; 0.02% NaN3), dissolved in Laemmli buffer, fractionated on polyacrylamide gels along with 5 10% of the supernatant from the IPs and the input.
Active fractions were further fractionated and assayed.
CSF peptides were fractionated into 30 fractions.
The gradient was then fractionated into 12 fractions [ 13].
Fraction S14 was further fractionated by RP-HPLC.
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