Sentence examples for fractional labelling from inspiring English sources

The mass isotopomer distribution of respective ion clusters of metabolites in samples from the two parallel cultivations were converted into summed fractional labelling (SFL) as described before [ 29].

#24 and #25, samples for intracellular metabolite analysis were withdrawn 28 h and 44 h after inoculation and processed for GC-MS analysis with subsequent calculation of Summed Fractional Labelling (SFL, % scale) of selected intracellular metabolite pools as described in the Materials and Methods section with a total of four re-samplings per time point.

Fractional labeling of CO2 was estimated with much higher precision of about ±0.001 to ±0.005.

The information contained in the extracellular mass isotopomers has a higher resolution compared to the summed fractional labeling used previously in [ 14].

Table  1 gives the corrected abundance of the mass isotopomers for hydrolyzed amino acids in 20% [U-C]GL labeling experiments; the mean fractional labeling (FL) was approximately 0.047.

Glutamine fractional labeling, mostly the M + 2 isotopomer, increased sharply at the end of the phase, when very little glutamine remained in the media and the contribution of secreted glutamine played a large role to the labeling state.

The flux distribution of any growth condition was calculated by the adopted model of Gombert et al. (2001) under the concept of summed fractional labeling (SFL) (Christensen et al. 2002).

This indicated GL was assimilated simultaneously with CO2 fixation in chloroplasts, resulting in different dilutions of FL values between amino acids synthesized in different cell partitions.> -wrap-foot> fractionalonalabelingng; SD, standard deviation.

From the raw GC-MS data, the summed fractional labeling (SFL) of each fragment was calculated as follow: SFL = 100 × [ 1. m1 + 2. m2 + …. + n. mn) × (m0 +  m1 +  m2 + … +  mn -1, with mn -1e fractional abundance of the lowith corrected moss and mi > 0 the abundance ofractionals with higher corrected mabundancee labelling data are available in request.

Newer studies rely on C-MFA applied by fitting the summed fractional labeling [ 14] or by fitting steady-state labeling data [ 15] resulted from using in parallel more labeled substrates for determining the intracellular fluxes at metabolic steady state in different growth phases.

For the KIX domain in the complexes KIX��MLL and KIX·MLL·pKID, stereospecific assignment of the prochiral methyl groups of valine and leucine residues were obtained using the 10% fractional labeling method of Neri et al. Intramolecular distance restraints were obtained from 3D H H-NOESY N/C-HSQC experiments.