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At the end of the transpiration measurements, fraction of transpirable soil water (FTSW) per day was calculated using the morning pot weight measurements and calculated as: FTSW = (Pot weight day n - Final pot weight)/(Initial pot weight-Final pot weight).
The relationship between transpiration and soil moisture was estimated for each variety by relating the normalized transpiration rate (NTR) to the fraction of transpirable soil water (FTSW) according to Sinclair and Ludlow [ 52].
The initiation of a decline in transpiration rate with soil drying occurs at certain fraction of transpirable soil water (FTSW) thresholds [2], which indicates a significant and exploitable water-saving trait.
Water stress was imposed by moderated decline in soil moisture and TR was analyzed relative to the fraction of transpirable soil water (FTSW); the TR inflection point provides a measure of a genotype's ability to regulate water loss under soil moisture stress.
Fraction of transpirable soil water.
Fig. 1 a Plot of transpiration rate of plant subjected to soil drying relative to well-watered plants versus the fraction of transpirable soil water (FTSW) remaining in the soil (adapted from Sadok and Sinclair 2011).
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