Sentence examples for fraction of interest from inspiring English sources

Thus, the redefined fraction of interest under deletion Df(1 ED6906 encompasses 124 kb between coordinates 7.09 and 7.21 Mb.

Additionally, by using HPLC detectors (refractive index, fluorescence, or UV), the elution range of the fraction of interest can be accurately determined.

Despite fraction collection under backflush conditions, which lowers selectivity of isolation of the fraction of interest, capillary chromatography of the isolated fraction allows accurate and precise quantitative determinations.

The fraction of interest was collected and then injected on straight-phase column, to separate the 13-HPOT and 13-HOT.

The fraction of interest was dissolved in 0.1% trifluoroacetic acid (TFA) and a reversed-phase high-performance chromatography was performed using a C2/C18 column (10 mm × 4.6 mm, 3  μm, 120 Å) (GE Healthcare), preequilibrated with 0.1% TFA.

However, a significant limitation of the technique is the problem of cross-contamination, in which small amounts of proteins from neighbouring compartments contaminate the fraction of interest [ 7, 21- 23].

The fractions of interest were analyzed, and proteins were identified by LC ESI-MS/MS.

After washing with PBS, we eluted the His-tagged proteins with a gradient of increasing concentration of imidazole (pH 7.0) and subsequent dialysis of the fractions of interest into PBS.

Not only is this at least an order of magnitude better than what can in principle be accomplished by conventional, single-stage solvent extraction, but such extraction is inapplicable to our system because of the insolubility of the pitch fractions of interest in typical liquid solvents.

A gradient of 0 60% acetonitrile/1% trifluoroacetic acid (v/v) at 1 ml.min−1 was established over 45 min, held for 5 min, raised to 80% acetonitrile/1% trifluoroacetic acid (v/v) over 10 min, and then returned to the starting conditions over 15 min. Fractions of interest were analyzed by MALDI-TOF/TOF MALDI-TOF/TOFetry (Bruker Ultraflex II).

STEP 3 – When needed, the fractions of interest were subjected to a final purification step on a narrow-bore reversed-phase column (Xbridge BEH130, Waters Associates) at 40°C at a flow rate of 0.25 mL/min using the biphasic gradients described in STEP 2. All HPLC purifications were performed on a Waters HPLC system (Waters 600 pump) equipped with a photodiode array (Waters 996 PDA).