Sentence examples for fraction extraction from inspiring English sources

These, are in particular, effective up to 65% 85% % organic fraction extraction (Shui et al. 2006).

Intracellular protein fraction extraction conditions were standardised, as well as the 2D electrophoresis.

Insoluble material, which remained after the membrane fraction extraction, was washed twice with 40 mM Tris pH 7.6.

After centrifugation at 20,000×g in an Eppendorff centrifuge for 20 min. at 4°C, the supernatant was saved as membrane fraction (extraction 1).

Protein extraction from whole cell lysates was as previously described, and nuclear fraction extraction and analysis by immunoblotting are described in detail in the supplementary methods.

In the case of the supernatant fraction, extraction started with 0.5 ml of the supernatant from the protein precipitation step onwards.

At the same time, almost all of MOMP was detected in the extracts enriched in membranes, membrane organelles, chlamydial organisms inside inclusions, and nuclear fractions (Extraction Buffers II and III respectively).

At the experimental end point, culture media were collected and stored at −80°C until cytokine concentration evaluation with ELISA and cells were lysed for cytoplasmic and nuclear protein fractions extraction.

The aqueous fraction after extraction was acidified with concentrated HCl to pH 2 and extracted again with three equal volumes of ethyl acetate.

Figure 8 μFT-IR spectrum of T2 sample performed on the soluble fraction after extraction with water.

For the available fraction an extraction of 30 min seems enough, as no further significant change was observed in the quantity of mercury extracted thereafter.