For each sample, fra-1 Ct value was subtracted from g6pd Ct value (Ct fra -1-Ct g6pd ), used to normalize the amount of cDNA.
Interestingly, contrary to Fra-1 mRNA expression levels, Fra-1 protein levels were significantly higher in Scp-2 cells than Scp-21 and MDA-MB-231 cells.
With no difference in Fra-1 protein degradation or mRNA levels, we measured Fra-1 tratesation rates.
Separately, miRNA-143 was also found to target Fra-1 mRNA, suggesting that this and other miRNAs are also candidates for Fra-1 regulation.
This predicts the BD of Fra-1 as capable of activating phospholipid synthesis when Fra-1 is expressed and should, in consequence, support normal growth rates.
C) Relative Fra-1 protein expression.
Therefore, Fra-1 expression increases motility.
B) Relative Fra-1 protein expression.
Fra-1 has many direct and indirect targets.
Cells with nuclear Fra-1 immunoreactivity were considered positive.
Next, we compared the differentially up-regulated genes between bleomycin-treated Fra-1∆/∆ and Fra-1 +/+ mice (Table 2).
