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In order to generalize the approach, a rational FP sensor methodology was established herein, by engineering instability in the secondary structure of an aptameric recognition element.
Furthermore, the FP sensor was embedded in the concrete structure for sensing the temperature change during the early age of hydration.
The design and fabrication of the FP sensor has been described previously in detail in [ 25].
Since the OCT probe and FP sensor interrogation beams share the same scan engine, it should be possible to straightforwardly integrate the FP sensor within most existing OCT scanner designs, including high speed parallel approaches.
To avoid this, a glass wedge was inserted between the scan lens and the FP sensor head during the OCT scan.
It also has the effect of introducing a small fixed lateral offset Δr between the OCT and FP sensor interrogation beam.
Similar(47)
Only a few existing single FP sensors are ratiometric, displaying two emission maxima at a single excitation wavelength.
The generally accepted mechanism of single-FP sensors assumes changes in the solvent accessibility and consequent quenching of the fluorescence (Akerboom et al., 2009; Chen et al., 2013).
Since the lifetime values in absence and presence of ammonium differed, at most, by less than 1 ns for AmTrac-LE and -GS we exclude external quenching effects due to altered solvent access in AmTracs, which is the widely accepted mechanism of single-FP sensors.
To our knowledge VSFP3.1 is the fastest FP voltage sensor that is functional in mammalian PC12 cells reported to date, exhibiting an activation time constant matching that of fast neuronal electrical signals.
The key elements of the system are a photoacoustic excitation laser system, a Fabry Perot (FP) ultrasound sensor for detecting the PA signals, a 1050 nm spectral domain OCT (SD-OCT) system and a galvanometer based optical scan engine that is used to spatially map the PA and OCT signals.
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