Importantly, a stably and homogeneously expressing Vpr-IN-FP cell line is not possible because of the known cytotoxic properties of Vpr.
Typically, both HOXA9 and HOXA10 are highly expressed in MLL-FP cell lines and show almost no expression in non-MLL-FP cell lines.
To further analyze the potential importance of RUNX1 in t(4 11) leukemias, we compared gene expression patterns and complex assembly at target genes in different MLL-FP cell lines.
To do so, a fragment spanning the sgRNA integration site within the LentiGuide plasmid was amplified from the gDNA of each clonal line (FP: 5′-CGTGACGTAGAAAGTAATAATTTCTTG-3′, RP: 5′-GTTATCAACTTGAAAAAGTGGCACCG-3′).
On the horizontal axis, we indicate the number of GPUs and on the vertical axis the time it takes to complete one BP (solid line), FP (dashed line) or one SIRT (dotted line) iteration.
The plasma frequency (fp) profile (black line and uncertainty bars) from 90 km to the F2 peak, derived from the vertical echo traces (e.g., Huang and Reinisch, 2001), is superimposed on the ionogram.
FP data (black line) were fit to a two-site binding model.
We conclude that we succeeded in developing, for the first time, an on-line FP-based HRS platform to screen for ERα affinity using the novel on-line FP detector cell recently described in a FIA mode by Kool et al. [ 20].
These wavelengths were therefore used to further optimize the on-line FP system in FIA mode.
The novel on-line FP detector cell can in principle be applied to other HPLC-based bioaffinity assays which require FP detection.
Finally, after optimization of the assay in FIA mode, the on-line FP detection system was coupled to a gradient HPLC system.
