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Exact(5)
Finally, a fourth fraction eluted with a second 2 mL of absolute ethanol, (SPE1-Fr4).
The activity bands from the fourth fraction of the two treatments were cut out and extracted from the native gel.
This fraction was then centrifuged at 240 g for five minutes and the bottom fourth fraction was collected [ 8].
The first fraction (F1) was eluted with pentane (100%), the second fraction (F2) with pentane: diethyl ether (50 50), the third fraction (F3) with diethyl ether (100%) and the fourth fraction (F4) with chloroform: methanol (93:7).
On analysing each fraction, no CYN was found in the first two fractions (8 ml of eluted solvent), but it started to elute in the third fraction and was completely eluted after the fourth fraction.
Similar(55)
Relatively high deviation of tebuconazole concentrations in the second fractions and copper concentrations in the fourth fractions were observed for the three parallel experiments at ECN Petten.
In control samples only the third and fourth fractions contained activities on both MUA and MUB.
Double derivatives were present also in the third and fourth fractions.
SDS- PAGE gels of a sample from the fourth fractions revealed a number of protein bands (Fig. 2C, Fraction panel).
This third fraction is the crucial factor.
He had the first fraction of a beard.
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