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It is the average of three 11-point numerical rating scales pain today, worst pain in the last 4 wk, and average pain in the last four wk.
Four wk after the initial immunization, the mice were boosted subcutaneously (first boost) with the same dose of conjugate, but with the substitution of incomplete adjuvant (which was also used in subsequent boosts).
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Gonadectomized wild type females showed an important decrease of relative bone volume (Fig. 4A) and trabecular number (Fig. 4B) four wks after surgery.
Good viability of L. plantarum M-13 was observed in PFOF over a period of three wk of storage at room temperature and with refrigeration.
Two wk after weaning, calves were blocked by age and randomly assigned to 4 treatments according to a randomized complete block design.
Twenty-four Ile de France ram lambs were evaluated in a 2 × 2 factorial design involving different DL-α-tocopheryl acetate injections (C, control = 0 and V = 150 IU/wk, i.m. for eight wk) and energy level (N, normal = 7.61 and L, low = 6.47 MJ/kg DM).
Eight wk old C57BL/6 mice were purchased from the Jackson Laboratories.
Rat splenocytes were isolated from five-to-six wk old female DA rats.
Four-to-five wk olocallylly bred LEW.1AV1 (RT1av1) or DA (B&K, Sweden) female rats were used in the experiments.
Cells were cultured in DMEM supplemented with 10% heat inactivated fetal calf serum, 1% pencillin-streptomycin and 1% L-glutamine (all from Invitrogen) for 24 h at 37 C. Four-to-five wk oLEW.1AV1AV1 or DA female rats were injected with 100 µl of 10 µM cardiotoxin (Latoxan, Rosans, France) in the gastrocnemii muscles.
Fifty-four wk old alphaA/BKO lenses were much smaller than similarly aged normal lenses, and were similar in size to 5 wk old wild type lenses.
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