Maybe the paradigm that HJs, single or double, comprise four uninterrupted strands is too narrow?
With works by Bacewicz, Tailleferre, Farrenc, Cecilia McDowall, Gabriella Montero and Rebecca Clarke also on the menu, sit back and enjoy an uninterrupted sequence from over eight centuries of musical talent.
Below a repeat number threshold [Messier et al., 1996; Rose and Falush, 1998], which varies across species [Balaresque et al., 2003] but lies close to seven or eight uninterrupted repeats, a microsatellite sequence is not considered to be polymorphic.
But by choosing my restaurants and dishes carefully, I stayed within budget and had an uninterrupted sequence of hugely enjoyable meals, five nights of pleasure I'd readily repeat.
Twenty-two (37%) of the sequenced clones contained microsatellites with at least seven uninterrupted repeats; 17 of these sequences were suitable for designing locus-specific primers with Primer3 (Rozen and Skaletsky, 2000).
I'm occasionally able to stomp a dozen or fourteen in a row under alternate feet in perfect, uninterrupted sequence, like a pool shark running the table.
Every match is treated by the commentators as an uninterrupted sequence of ecstatic climaxes.
That is, the expanded allele is comprised of an uninterrupted sequence of CAGs encoding glutamine.
For both sequences this allowed the reconstruction of two uninterrupted, contiguous haplotypes from each inbred line, presumably corresponding to the two sub-genomes, spanning just over 900 bp in wcd-12699 and 2.3 kb in wcd-2781.
Among the 33,290 sequences obtained, a total of 201 sequences longer than 80 bp, containing microsatellite motifs with at least seven uninterrupted repeats and flanking regions free of tandem repetitions, were used to design PCR primer pairs.
The remaining 397,224 sequences were split into four groups (due to computer memory constraints), and the first group of 99,999 sequences was searched for perfect di- to hexanucleotide microsatellite repeats with a minimum of seven uninterrupted repeat units using a search tool in Geneious (Phobos plugin; Mayer, 2010), which identified 484 repeats.
