Finally, the spectral ratio was averaged for the seven time sections.
Next, Fourier amplitude spectra for these seven time sections were calculated with a Parzen window of a band width of 0.05 Hz.
Here, although the averaged H/V spectral ratios for different measured time are calculated from seven time sections of 163.84 s extracted from the observed microtremor data, we have confirmed that same value of the peak frequencies is shown in the all seven time sections.
Then, a microtremor H/V spectral ratio was calculated for each of the seven time sections, where the mean of the horizontal two components was adopted as the numerator.
The procedure to calculate the H/V spectra can be summarized as follows: First, a high-pass filter of 0.1 Hz was adopted, and seven time sections of 163.84 s each were extracted from the original data considering recorded noise.
Fischbacher had the fastest split times in the final three timed sections of the course, and her time to the midway point ended up being 0.10 of a second ahead of Vonn's.
After washing with PBS three times, sections were incubated with goat anti-rabbit IgG-horseradish peroxidase for 30 minutes.
Following washing with PBST (containing 0.1% Triton-×100 and 0.5% BSA) and PBS three times, sections were treated with horseradish peroxidase (HRP) conjugated goat anti-rabbit antiserum (Jackson laboratories, PA, USA).
After washing with PBS three times, sections were incubated with a secondary anti-mouse horseradish peroxidase conjugated antibody for 30 minutes at room temperature and washed in PBS.
After washing with PBS three times, sections were treated with 2 N HCl for 10 min at room temperature, followed by three washes with PBS, and incubated with anti-BrdU antibody conjugated with peroxidase at 37°C for 2 h.
