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The RT-PCR analysis resulted in weak amplification (positive) from the first lysate, but no amplification was found in lysates of four passage cells.
RNA samples were extracted and purified from 150-μL lysates of primary cells and four passage cells and used as templates for RT-PCR analysis to determine the presence of TSV.
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NSs maintained a significantly higher proliferation rates in the HMF condition in four passages.
After four passages, E. agglomerans survivorship increased from 2 days to 2 weeks.
Chondrocytes were isolated from rabbit ear cartilage and grown in monolayer cultures over four passages.
Moldrich et al., (2009) used cells after at least four passages in culture, whilst we subjected cells to only one passage in culture before conducting the microarrays.
HUVEC were analyzed with four passages.
2006p00149 after ∼2 weeks (two to four passages) of culture.
For the following experiments, cells at four passages were used.
After four passages, the cells were served for cloning.
Strain Br1622 has undergone from four to six passages before its deposition, and a further four passages for the final volume of inocula.
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