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A plot of the frequencies of the four nucleotides as a function of position with respect to the poly(A) site is a useful tool for assessing PAC and PAT quality; accurate demarcation of poly(A) sites is expected to yield defined poly(A) signals, while random localizations of mapped PATs is expected to yield a uniform, unbiased nucleotide distribution in the regions of PACs.
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The retention of nucleotides was investigated as a function of methanol content in the mobile phase.
As a result, each nucleotide is formulated as a function of S and R (Equations 1-4).
Thereafter in Finnish strains nucleotide changes occurred linearly as a function of time.
The counts were averaged in a 60 nucleotide window that slid 5 nucleotides per step to generate a continuous curve of VWG/CWB counts as a function of nucleotide number.
If this is indeed the case, it would account for the different order of amplitudes as a function of nucleotide for the two types of fluctuation.
No simple model exists that accurately describes the melting behavior and breathing dynamics of double-stranded DNA as a function of nucleotide sequence.
10.7554/eLife.02384.009 Figure 3. Pol V Mut binding and activity as a function of nucleotide.
Concentrations of dUMP and dTMP were determined from standard curves of peak area as a function of nucleotide concentration.
To calculate the bimolecular rate of nucleotide binding, 1 μM H115W was mixed with incremental amounts of one of four dNTPs, in the presence of 10 mM free Mg2+ in both solutions, and the rate of fluorescence change was measured and plotted as a function of nucleotide concentration.
A gel showing primer utilization (%PU) as a function of nucleotide is presented to the right of the graph.
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