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Processing was started within four hours of sample collection.
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All aliquots of the blood samples were stimulated within four hours of sampling.
This test can diagnose TB within four hours of sampling by detecting anti-MA antibodies as immune surrogate markers for active disease even in HIV infected patients.
T lymphocyte estimations were performed within eight hours of sampling.
PBMC were isolated within two hours of sampling using CPT tubes (Becton Dickinson, Franklin Lakes, NJ, USA).
Serum was pipetted in aliquots and frozen at −80 °C within two hours of sampling.
Within six hours of sampling, the cartridges were transported cold (4°C) to the laboratory.
Plasma was separated and stored at –80°C within six hours of sampling for subsequent analysis in batches in a specialized hematology laboratory.
After four hours of stimulation samples were washed twice, first with 500 μL, then with 1 mL PBS supplemented with 0.1% sodium azide (NaN3) and stained for flow cytometric analysis.
We also recorded subsequent "dyads" of body temperature and serum phosphorus levels if available; we required that the temperature have been recorded within four hours of the time that the serum sample was obtained.
However, the mean H2O2 content in CVF samples after four hours of full exposure to air was still only 23 ± 5 μM, one hundred times lower than maximal aerobic in vitro production (~2 mM).
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