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The relative abundance of each gene in the mRNA pool was deduced by determining the Trimmed Mean of M-values (TMM) normalized number of reads mapped in the exon region and classified into four expression categories: Very highly expressed (read count > = 1000), Highly expressed (1000 > read count > = 100), Medium expressed (100 > read count > = 15) and Low expressed (15 > read count > 5).
K-means clustering of these differentially expressed genes identified four expression patterns that reflected the progression of the disease with the magnitude of the fold-change being higher in infected 66VO fish.
A total of four expression constructs were prepared, heterologously expressed, the products purified, quaternary structure investigated and crystallization trials carried out.
The initial screening of bispecific antibody pairs was conducted using crudely purified antibodies expressed in HEK293T cells co-transfected with a mixture of four expression vectors encoding the heavy and light chains of anti-FGFR1 and anti-KLB IgG as described in Supplemental Materials and Methods.
We showed that these four expression cassettes can be combined in a single lentiviral vector without causing recombination.
We developed a computational method to infer the probability of AH and applied it to three GWASs and four expression quantitative trait loci (eQTL) datasets.
In this paper, we report how switching from a trial and error to a fractional factorial approach allows testing in less than 2 weeks four expression variables (E. coli strains, culture media, expression temperatures and N-terminal fusion tags) in a single experiment.
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The Japanese have four expressions for inebriation.
He said there had been four expressions of interest.
Four expressions of Gmax from the literature are examined.
Each set contained four expressions, one for each operation.
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