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Seven tet-o-PyMT-IRES-Luc transgenic founders (#1, 2, 20, 21, 23, 29, and 39) were identified by PCR genotyping among the 39 pups obtained from the microinjection of this transgene into C57BL/6 mouse eggs, and the transgene was transmitted to the progeny of all 7 founder lines.
Five transgenic founders (#1, #2, #4, #33, and #37) were generated and identified based on a PCR analysis of their genomic DNA.
We measured four fitness components among the founders: 1) male and female reproductive success using Y-chromosome and mitochondrial genetic markers; 2) individual reproductive success using parentage analysis; 3) social dominance and competitive ability using RFID tags; and 4) survivorship.
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At least five specific mutations in these genes have been found to recur in cancer families of French Canadian descent [ 2- 5] and this has been attributed to common founders [ 1, 3, 6- 9].
Like Y Combinator and TechStars, YEurope invests in startups based on the number of founders (1-3, 5000€/eand), and taking a 2-102-10%ke in the company.
In the constitutional system envisioned by our founders 225 years ago, individuals get one vote.
Two founders were randomly selected for each transgene and crossed with C57BL/6J wild type mice: for Trpc6-HA wild type founders 419 and 421, for Trpc6-HA P111Q founders 615 and 616, and for Trpc6-HA E896K founders 73a and 75a.
The transgenesis procedure yielded 7 founders, 5 of which expressed LacZ activity during embryonic development.
Germline transgenic founders (F0) were identified by screening F1 embryos via PCR.
Among CC founders, 92 miRNAs were differentially expressed.
Founders sequenced: Estimated heterozygosity from a pedigree with sequenced founders 19.
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