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Here in a concrete building west of Sukhbaatar Square, quotes from the nation's founder line the walls.
We focused our studies on founder line 17.
The BXB23 founder line has been used extensively in genetic studies to analyze tumor progression influencing factors [7] [12].
10 founder lines were expanded for further characterization, and the founder line with the highest level of expression was then used for all subsequent experiments.
However, founder line #20 expressed luciferase ubiquitously without a tetracycline regulatory protein, as determined by in vivo bioluminescent imaging (data not shown).
Line A and line D [6], [20] are the more widely used founder lines in comparison to the number of studies that have utilized founder line F [21].
Based on these results we decided to focus on a founder line with an intermediate transgene copy number (#1), which is termed Col1a1-Krm2 for the remainder of the manuscript (Figure 2B).
In founder line #29, 21/34 bi-transgenic iBRAF* males on doxycycline (ON) developed prostate tumors with an average latency of 24 weeks (SD = ±6 weeks) (Figure 2B, Supporting Table S1).
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One of the founder lines, designated PAR39, developed an early onset cataract that involved formation of a plaque of cells at the anterior aspect of the lens.
Three founder lines from both transgenic strains were selected for further analysis.
The BC2F1 plants representing all the founder lines are generally used for developing BILs.
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