Synthetic, complementary DNA single strands and short interfering RNA double strands have been found to inhibit the expression of animal, plant, and viral genes in cells, animals, and patients, in a dose dependent and sequence specific manner.
In a previous study, A77 1726 was found to inhibit the expression of monocyte-activating factor at the surface of T lymphocytes, which in turn decreased the activation of monocyte/macrophages, and thus their production of IL-1β and matrix metalloproteinase (MMP -1 [ 16].
In the present study, TPZ was found to inhibit the phosphorylation of mTOR and 4E-BP1, which accompanied the loss of HIF-1α expression.
Treatment with compound 44 was found to inhibit the activation of mTOR downstream target proteins p70S6K and eIF-4E1 and inhibit HIF-1 α expression.
To inhibit the expression of p600, we used p600-targeting siRNA, and we found that p600 expression was significantly inhibited for 5 days after transfection with p600-targeting siRNA (Fig. 2a, b).
ITCs have been shown to inhibit the expression of estrogen-responsive genes.
Furthermore, Heps have been found to inhibit expression of oncogenes and to affect the immune system (Smorenburg and Van Noorden, 2001).
Polyphenols have been found to inhibit expression of endothelial adhesion by regulating gene transcription (Carluccio et al. 2003).
Accordingly, the restoration of p53 and/or CDKN2A expressions was found to inhibit MMPs expression and tumor cells invasion [6], [8] [12].
PqsR was also found to inhibit antABC expression, albeit in a PrrF1,2-independent manner (Oglesby et al., 2008).
Sudachitin had been found to inhibit NO production by suppressing the expression of iNOs in LPS-stimulated macrophages, to exhibit anti-inflammatory activity, and was a more potent anti-inflammatory agent than nobiletin [67].
