Second, that Mad was found to be phosphorylated in a segmental pattern and to be required for segmental patterning.
Approximately 49.6% of the phosphopeptides identified was found to be phosphorylated in a single site, and other fractions were either phosphorylated doubly (34.4%), triply (15.3%), or at more (0.8%) sites.
However, it has to be noted that there is no evidence that residues that were found to be phosphorylated in H. salinarum and are conserved throughout a variety of other species, are also phosphorylated in these species.
Of the phosphorylated RTKs measured, the erbB family members were found to be phosphorylated in DiFi parental cells and in DiFi62 and DiFiG cells.
Intriguingly, this peptide contains Ser and Thr, which we found to be phosphorylated in our structural characterization of AF4.
Paxillin, in contrast, was found to be phosphorylated at Tyr118 when membrane associated (Fig. 4b) and localized near ITGαVβ3 exclusively in AT-cells (Fig. 4c, arrowhead).
Among 14 phosphorylated proteins involved in transcription and translation in H. salinarum, only one archaeal transcription regulator, sirR (OE1797R), which is conserved in archaea, was found to be phosphorylated on four possible residues: Thr26, Ser27, Thr28 or Tyr32.
However, while in S. aureus either Tyr440 or Tyr444 are phosphorylated, the IsdB protein expressed in L. lactis was found to be phosphorylated on Tyr311.
A variety of proteins have been found to be phosphorylated by activated IR.
In our study, both NHERF2 Ser and Thr were found to be phosphorylated.
In HeLa cells treated with 100 nM staurosporine, we found PDK1 to be phosphorylated at Ser241 in R21A-expressing cells.
