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This SNP was found to be monomorphic in our population and was further not analysed.
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SNPs found to be monomorphic in the AFDS (n = 9) were not analyzed further.
A total of 60 SNPs were found to be monomorphic in the tested samples, yielding a conversion rate of 51% (Table 2).
Notably, locus Lpu15 was found to be monomorphic in varieties pumila and lanceolata.
The risk alleles in the gene, however, were found to be monomorphic in two Asian populations examined: Japanese and Korean.
Besides the 11 polymorphic microsatellites, 2 other markers were found to be monomorphic in the group of Newfoundland dogs, but might be polymorphic in other breeds.
Ten of the 13 SNPs found to be monomorphic in the laboratory strains were polymorphic in natural populations with frequencies ranging from 0.9 to 12.1%.
Curiously, 25/23 and 1/1 polymorphisms reported in HapMap and Perlegen, respectively, were found to be monomorphic in similar populations that we examined.
We also could not verify significance of 4 SNPs in the ZC3H12a gene which were found to be monomorphic in this sample set.
However, about 20% of a total of 909,622 SNPs were found to be monomorphic in the Japanese population, which is due to SNP selection methods.
The majority (91%) of predicted SNPs that were found to be monomorphic in the present study have their minor allele represented by 2 reads only.
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