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We found that the GC-content of the turtle transcripts was lower than expected for other reptilian EST efforts [ 39].
In line with our hypothesis that MNase digestion coupled to size selection leads to an enrichment of GC-rich sequences, we found that genome wide the GC-content of the DNA fragments recovered after MNase digestion of naked DNA exhibit an increase of GC-content in the fragments like the in vitro and in vivo experiments.
For example, we found that the probe sequence GC-content affects the extent of cross-hybridization and may affect cross-hybridization in different ways, depending on the type of alignment between probes and transcripts.
In contrast, we found that the GC content of E. cymbalariae with only 40% is similar to that of S. cerevisiae (38.3%) and other hemiascomycetous yeasts.
We found that the GC content of the HGT candidates is not different from the rest of the genome (p = 0.337).
Firstly, we found that the GC content of the PAI region differed from the remaining genome.
We found that the GC content in phages correlated strongly with the GC content of the host (correlation coefficient r = 0.89, P ≪ 10-15, Figure 5).
In the uncovered bases of Illumina GA, we found that the GC content was higher than that of the genome of E. coli DH1 ME8569 strain (50.8%).
In addition, it has been suggested that the GC-content of the transcripts could influence the correspondence between platforms [ 26].
The GC-content of the PptAPx transcript is with 52.0% increased if compared to AttAPx transcript (46.3%) and to the average GC-content of Physcomitrella genes (31.7 - 47.7%; [ 48]).
Since H-NS is strongly associated with regions of low GC-content [53], [54], operons associated with 1 kb regions that had a GC-content of less than 55% were combined with those found by the pattern matching.
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