In various organisms, the U6 promoter is used to express sgRNA; however, in Dictyostelium, we found that the expression level of sgRNA under the control of U6 promoter was lower than that achieved using tRNA.
In the present study, we found that the expression level of PPARα gene was significantly decreased in the APFL group compared with the AP group.
We found that the expression level of TLR4 (Fig. 4A) and the amount of TNFα produced upon LPS stimulation (Fig. 4B and 4C) were similar between CD56+ DCs and mDCs.
When assessing the activity profiles of the enzymes involved in pyruvate catabolism in Clostridium thermocellum (Rydzak et al. 2009), it was found that the expression level of the POR enzyme remained constant as growth progressed from early exponential to stationary phase.
We found that the expression level of all these postmeiotic phase markers were significantly reduced in the mutant testes compared to the controls (Fig. 2C).
We furthermore found that the expression level of FOXP3+ in CD3+CD4+CD25highCD127low/− cells was similar in AAV patients and controls.
By qRT-PCR, we found that the expression level of ABCA3 in SALL4 overexpressing cells was increased by 33% compared to those of the controls (Figure 5A).
In the present study we investigated the expression of miR-143 in prostate cancer and found that the expression level of miR-143 was significantly decreased.
Interestingly, when the two mutations (Y111C and K47N) present in Swi3-E68 were characterized individually, we found that the expression level of Swi3-Y111C was lower than wild-type and that Swi3-Y111C failed to interact with Swi1 (Figure 2B).
Finally, we found that the expression level of core protein could be reflected by the activity of Fluc in the mouse model, and shRNA targeting HCV core protein could effectively downregulate core gene and Fluc gene expression in vivo.
We found that the expression level of ATHB12 was correlated with the severity of abnormal development in C4 transgenic plants and that infection of Arabidopsis with a BCTV c4 mutant did not induce significant symptom development or ATHB12 gene expression, implying a relationship between the functions of the BSCTV C4 protein and the effects of ATHB12 induction on symptom development.
