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Tumour cells were treated for 72 h (i.e., the time we found required for maximal downregulation of SCGB2A1, based on qRT PCR), after which they were used as targets in cytotoxicity assays as described below.
USC cells were treated for 72 h (i.e., the time we found required for maximal downregulation of CD46, CD55 or CD59, based on flow cytometry and qRT PCR), after which they were used in CDC and antibody (trastuzumab -dependent cellular cytrastuzumab -dependentescellularelow.
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Most of the examples we found required substituting space for time and thus constituted weaker inferences.
Screens of deletion mutations, called deficiencies (Dfs), each of which removes multiple genes, have also been used to find genes required for embryonic development.
Another potential use of Df screening would be to find genes required for normal cellular or subcellular expression of proteins that can be visualized by antibody staining.
In keeping with this, we find Psy2 required for Maf1 dephosphorylation, but not Psy4.
In several non-neural stem cell types and in germ cells, microRNAs have been found to be required for stem cell maintenance (for review, see [40]).
Given that Rlf was found to be required for normal DNA methylation status at regulatory elements throughout the genome, we were interested to find protein partners.
This region is found to be required for Prp19 function and yeast viability.
The intact cytoplasmic C-terminal domain was found to be required for ligand-induced APJ internalization.
The dissolution of the zinc substrate is found to be required for the precipitation of silicate-rich surface layers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com