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The injected blastocysts were introduced into foster mice.
Loss of high-frequency sound hearing in Asic3−/− foster mice implies that they may have problems communicating with pups and fail to fulfill the pups' needs.
The method described here substantially diminishes the number of oocyte injections and foster mice and thus increases the throughput compared to single construct injections [19].
DNA fragments for generating transgenic mice were released from the respective constructs using PvuI and NruI/EcoRI restriction endonucleases, diluted to 3 ng/µl, and injected into the pronuclei of FVB oocytes [51], which were subsequently transferred into the oviducts of 0.5 day, pseudopregnant, CD1 foster mice.
Two independent subclones were then injected into blastocysts and transferred to foster mice.
In addition, some foster mice implanted with morulae injected with FVB/n or FVB/n;129/Ola ESCs were unable to give natural birth and caesarean section was required.
Similar(53)
BALB-foster mice demonstrated a greater occurrence of B to B self-transitions and a lower occurrence of A to A, A to B, B to A, A to Z, and Z to A transitions compared to sons of B6 and B6-foster mice (p<0.05, Fig. 6).
This change also necessitated adding a group of SD-foster mice, who mated during the KD and SD mating days, and who adopted the pups from the study groups.
Foster foster foster!
Foster female mice (F1) were crossed with ligated male mice 0.5 day before embryo transfer operation.
Foster mother mice were euthanized on E15.5, E16.5 or E17.5 to screen potential transgenic embryos for cleft palate.
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