For these markers, the forward marker-specific primer had a T7 universal sequence (5'-TAATACGACTCACTATAGGG-3') added to its 5' end.
The wide choice of potential and proposed antigens, routes and mechanisms of delivery, and possible treatment regimens suggest that, to move the field forward, surrogate markers allowing comparison of the relative efficacy of different vaccine approaches are required.
Ferguson will be a milestone -- good or bad -- in our country's history of relationships and I have a mind, for whatever small part I play in it, to make it a forward-moving marker on my personal timeline.
Moving forward, more markers are absolutely essential.
For creating multivariate linear QTL models, NEO also implements forward-stepwise marker selection.
The forward-stepwise marker selection method may avoid a pitfall that plagues the greedy SNP selection: if several genetic markers are located very close to each other (and are highly correlated), the greedy SNP selection may pick all of them before considering SNPs at other loci associated with the same trait.
Why not just spot the ball to the nearest yard marker, forward or backward?
Then, in the one-hundred-and-thirteenth minute, André Schürrle, one of Germany's substitutes, picked up the ball on the left wing and raced forward, escaping his marker.
Having picked up the ball from Nabil Bentaleb, the forward turned his marker then drove forward before guiding a low shot into Zengin's bottom-left corner.
Looking forward, no clear marker has materialized as a reliable predictor of traztuzumab resistance in the adjuvant setting.
