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The reverse amplification primer is unable to bind to P2 unless the complementary sequence is filled in during the first round of forward elongation originating from the P1 amplification primer.
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We suggest that the elongation and forward extension of the tubules result from the combined effects of cell rearrangements that lengthen the tubule and the response of kink region cells to regional Dpp guidance cues.
The distal tip of each tubule lies more posteriorly but moves forward as tubule elongation progresses.
Inelastic scattering experiments also revealed a few interesting features.4 It was shown, for example, that the translational H atom energy was transformed into a vibrational D2 energy mainly via a bond elongation with forward scattered products rather than bond compression and backward scattered products.
Cellular RNA polymerases are thought to be ratchets that can push each other forward to reduce pausing during elongation.
Xia et al. [11] put forward the translation initiation and elongation conflict hypothesis to explain the unusual usage of the CAU anticodon in tRNAMet.
When calpain activity is blocked, the cell has difficulty in detaching so that, as the front of the cell moves forward, there is trailing edge elongation and loss of the uropod structure.
In addition, Mfd rescues arrested or backtracked transcription elongation complexes by promoting forward translocation of RNA polymerase in ATP dependent manner leading to productive elongation [13].
During translation elongation, the protein faces forward, toward the 3′ end of the mRNA [ 44, 45].
Since loop 3 is unlikely to directly interact with elongation factors, they put forward a hypothesis in which this loop assists in pseudotranslocation by affecting ribosome conformation.
Tip cell anchorage antagonizes forward-directed, TGF-β-guided tubule elongation, thereby ensuring the looped morphology characteristic of renal tubules from worms to humans.
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