We assume that there are fortuitous sequences upstream of the g27 gene in pMS98 that resemble both promoter and ribosome binding sites for integrase expression after conjugation to Streptomyces.
Following this argument, DS patterns under the PGD hypothesis would ultimately arise as a consequence of a highly fortuitous and unlikely sequence of rearrangement events.
Extensive sharing of traits in closely related lineages could be erroneously interpreted as a highly fortuitous and unlikely sequence of events.
On Discussion paragraph 4 (page 8) Martin et al. admit that it could be argued (as I do) that under the PGD model the double-synteny patterns "would ultimately arise as a consequence of a highly fortuitous and unlikely sequence of rearrangements".
Expression of the active thermostable esterase in E. coli was independent of isopropyl-β-d-thiogalactopyranoside and of the kind of vector, suggesting that the archaeal esterase gene was controlled by fortuitous bacterial-like sequences present in its own 5′ flanking region, not by the bacterial lac promoter or other serendipitous vector-located sequences.
All gene segments less frequent than TRBV5-7 (withethexceptionof of TRBV3-1 itself) are currently annotated as pseudogenes, and all chromosome 9 orphon gene segments are in this group (we presume that the alignment to orphons is due to fortuitous PCR or sequencing errors derived from their paralogs on chromosome 7).
Prof Crisanti said his team exploited a "fortuitous coincidence": the target sequence of that endonuclease is found specifically - and abundantly - on the mosquito's X chromosome.
Briefly, PolyA_DB collects candidates of human PAS by stringently aligning 3'ESTs and full-length cDNAs with apparent polyA tails to the genome, while ensuring that the presence of the polyA tail cannot be explained by fortuitous complementarity to genomic sequence.
When faced with evidence not fitting with this view, such as de novo genes, this view forces us to argue that it is still just a small genetic change that arises by a sequence of fortuitous chance events absent selection (a small whole gene, in this example), and that it could happen by accident after all (we will see if this approach is valid below).
