Cells were grown in SMC Growth Medium 2 (PromoCell, Heidelberg, Germany) and subcultured up to the forth passage (P4).
Cells were used for experiments prior to their forth passage.
Isolated stem cells were characterized through immunocytochemistry staining with monoclonal antibodies. 2 × 10 cells at forth passage were seeded on Lab-Tek Permanox chamber slides for 3 days and were fixed with 4%% paraformaldehyde solution in PBS.
In the wondrous slow movement, they were like jazz musicians in the back-and-forth passages.
At times, a character's speculation comes forth in passages that, for good or ill, might have been written by Jack Kerouac himself.
In two minutes, he can hold forth on passages from the Talmud, his custom-made pool cues, meals with Jesuits in a refectory and his conquest of Park Avenue, one matron's bedroom at a time.
Soon Mr. Aimard was switching back and forth between passages from a Scarlatti Sonata in G minor and a Beethoven Bagatelle in G major, music of strikingly similar character and texture.
The samples were considered negative after the forth-blind passage.
When I'm not working, I let my memories flow or call forth my favorite passages from Tacitus.
In one video, he contrasted his former self, covered in tattoos and speaking street slang, with a new man, one now covered in a prayer shawl, rocking back and forth over Torah passages.
These "forth and back" passages of tRNA recognition reflect main steps in evolution of the code mediated initially by aminoacylating ribozymes and then by their protein successors.
