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The release profiles of in situ gelling formulations were treated with the Korsmeyer-Peppas equation, and slope values n > 0.89 were indicating anamolous drug release involving a combination of both Fickian and non-Fickian diffusions through the Millipore membrane filter and excised goat cornea.
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In addition, EILDV-conjugated micelles inhibited the migration of B16F10 cells effectively compared with plain ETO and non-conjugated micellar formulations when cells were treated with equivalent cytotoxic concentration of the drug, i.e., IC25.
To investigate the induction of therapeutic efficiency of the formulations, Y-79 cells were treated with native etoposide, void nanoparticles (without drug) and NPs at different concentrations for 5 days, and cell proliferation was measured by a standard MTT colorimetric assay.
LNCap cells were treated with formulations and incubated for 24 h, and cell viability was determined by MTT assay.
The cells were treated with respective formulations and left untouched for 24 h.
After 24 h incubation, the cultures were treated with the formulations.
Cells were treated with different formulations at a concentration of 1 μg miRNA in serum containing medium at 37°C for 72 h.
SH-SY5Y cells were treated with all formulations (4 μM curcumin) and generation of ROS was determined at predetermined time points of 3, 6, 12, 24, and 48 h.
Then the cells were treated with nanotube formulations along with controls.
The cells were treated with respective formulations (at an equivalent concentration of 1 μg/mL) and further incubated for 18 h.
Fibrosarcoma was induced in mice by injecting benzopyrene (BAP) and tumor-bearing mice were treated with various formulations of etoposide, including etoposide entrapped camel milk phospholipids liposomes (ETP-Cam-liposomes) and etoposide-loaded DPPC-liposomes (ETP-DPPC-liposomes).
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