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Thus, the selected formulations were placed on long-term stability and also evaluated for their stability in response to mesh nebulization.
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A small amount of the formulations was placed on a glass slides, covered with a cover slip, and examined with the aid of a polarized light microscope (Jenamed 2, Carl Zeiss-Jena) to judge the homogeneity of the dispersions and the presence of optically anisotropic areas by the patterns formed with the sample between crossed polarizers.
Samples containing the same formulation were placed at the same time in an autoclave, exposed to mediums with supercritical CO2 or saturated in water and removed after 30 days.
For TEM study, some droplets of a well-dispersed sample of optimized formulation were placed on carbon-coated copper grid and then air-dried at room temperature.
One milliliter of formulation was placed on the upper surface of slide.
The diluted nanoemulsion formulation was placed on a carbon-coated copper grid supported with formvar films and allowed to stand for 2 min.
About 500 μL of each formulation was placed in the upper chamber, and the tubes were then centrifuged at 5000 rpm for 30 min.
Briefly, 1 ml of Lf-coupled SLNs containing about 100 mg of SLN formulation was placed in a volumetric flask with 1 ml (10%%) Coomassie blue G dye solution, and the volume was adjusted to 10 ml with distilled water.
Briefly, two mL of S6S-GNC formulation was placed inside a dialysis bag (MWCO 100 kDa, Fisher Scientific, USA).
The test formulation was placed between the two coverslips balanced on the left pan of the balance.
Five mL of the optimized formulation was placed in dialysis membrane and the sack was immersed in 50 mL of phosphate buffer solution (pH 7.4).
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