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More than 90% of the some nano-sized CFA formulations were dissolved in 3 min and complete dissolution occurred within 20 min, while the commercial CFA did not achieve complete dissolution (only about 50%) during 60 min of the testing period.
The RUB formulations were dissolved in chloroform/methanol (v/v, 9 1) and be deposited onto the subphase of the minitrough (KSV, Finland) using a microsyringe.
To determine the amount of total flavonoid, the formulations were dissolved using acetonitrile to extract the flavonoid content.
Drug formulations were dissolved or suspended in distilled water and analyzed with the flow-through optrode without further sample processing.
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The lipids for each liposome formulation were dissolved in 3 ml of chloroform to give 16.67 mM of total lipid concentration (GdSL1, 18.97 mg/ml; GdSL2, 16.97 mg/ml; GdSL3, 15.17 mg/ml; and SL, 13.41 mg/ml) and dried to a thin film using a rotary evaporator.
For measurement of swelling ratio, each formulation was dissolved in PBS (pH 7.4) at 37 °C.
Each Kampo formulation was dissolved at 1/1,000 of the daily dose (1 Unit), as indicated on the package insert, in 1 mL of purified water, and extracted by sonication at room temperature for 15 min.
For a typical formulation, 225 mg of ZnSO4 were dissolved in 0.25 ml of distilled water and emulsified in 2.5 ml of CH2Cl2 (containing 50 mg of a mixture of PLGA 503 H and BBB-PLGA (80∶20 w/w) (with or without 5 10 mg TMR-PLGA) by sonication over an ice bath using a probe sonicator (Misonix, MicrosonTM Ultrasonic Cell Disruptor XL, Opto-lab, Concordia, Mo, Italy) at 60 W output for 45 sec.
All drugs were dissolved in DMSO except decitabine, which was prepared in water for liposomal formulation.
Parliaments were dissolved.
To further enhance the formulation, collagen was dissolved into the binder solution to fabricate collagen-calcium phosphate composites.
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