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Optimized formulations were calculated and the optimized output values were in accordance with experiment results.
The IC50 values of various formulations were calculated by plotting a graph of percent cell viability vs. concentrations.
From the feed drug and copolymer blend, the theoretical drug loading contents (DLCtheoretical) of all blend nanoparticle formulations were calculated from Equation 1, giving the result of 6.25%.
Diffusion parameters of three different SIBS formulations were calculated and used to predict the behavior of a fourth type based on molecular weight and relative polystyrene content.
The area under the curve (AUC), Cmax, and Tmax for the test and reference formulations were calculated using the statistical moment method, and the related pharmacokinetic parameters are shown in Table 1.
Asymptotic sampling variances for all ten formulations were calculated using the formulae in Table 1.
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Colonies having more than 50 cells were counted, and the percent colony inhibition (PCI) capacity of the formulations was calculated as follows: Number of colonies in UC untreated control PCI = − Number of colonies in formulations Number of colonies in UC × 100 Open image in new window.
The enhanced oxime-transport using the nanoparticulate formulations was calculated related to the transported free oximes and was expressed as transport difference.
The AUC0-24h (for IB formulation) or AUC0-48h (for ibuprofen-OHB formulations) was calculated using linear trapezoidal rules.
The entrapment of S6S within the developed formulations was calculated using the following equation: (1) Entrapment Efficiency (EE % ) = Total S 6 S added (nM ) − Free S 6 S (nM ) Total S 6 S added (nM ) × 100.
Percentages of 1.1% (chemical standard) and 0.6% d-MTX (pharmaceutical formulation) were calculated by solving the above equations for y = 1.
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